The key to an optimal PCR experiment is primer design. In general, primers should be between 18 and 24 nucleotides long and have a GC content between 40 and 60 percent. Ideally primers should contain ...

GeneFisher—fishing for genes, perhaps? Fishing genes out of genomes? Just fishing? The subtitle, Interactive PCR Primer Design, “hooked me” (get it?) but it shouldn’t have in retrospect. After all, ...

The video above has some helpful tips for designing PCR primers for one purpose - cloning with restriction enzymes. PCR or Polymerase Chain Reaction, is a very common tool used in molecular biology to ...

Nematode-trapping fungi, a monophyletic lineage within the Orbiliales (Ascomycota), use specialized structures to capture and consume nematodes in soil, leaf litter, and other substrates. These fungi ...

Genome sequencing has become a powerful tool for studying emerging infectious diseases; however, genome sequencing directly from clinical samples (i.e., without isolation and culture) remains ...

Premier Biosoft is a long-time molecular biology software developer that has specialized in interesting niche products. Many of them relate to PCR and primers, and they are available for purchase. It ...

New England Biolabs (NEB®) today announces the release of the NEBNext Flu A Integrated Indexing Primer Module (NEB #E3436), ...