In recent years, quantitative real-time PCR tests have been extensively developed in clinical microbiology laboratories for routine diagnosis of infectious diseases, particularly bacterial diseases.

Real-time PCR plays a foundational role in gene expression studies, disease analysis, and molecular diagnostics. However, success depends on precise reaction setup, optimized assays, and accurate ...

Real-time PCR is an important tool for life scientists looking to evaluate gene expression. While the chemical reaction underpinning the technique is relatively simple, real-time PCR instruments can ...