Figure 1: A schematic diagram of the use of fusion PCR to synthesize a fragment for creating a fusion of a sequence encoding the mRFP to the 3′ end of the histone H1 gene. We also describe a procedure ...

Here, we describe a method of systematic PCR screening with multiround sample pooling for the isolation of rare PCR-positive samples. As an example, we have applied this protocol to the recovery of ...

分子诊断主要是应用分子生物学方法检测生物体内遗传物质的结构或表达水平的变化而做出诊断的技术。与其他主要的体外诊断方法相比，一方面，由于分子诊断可以从基因层次进行检测，因此在检测灵敏度和准确性上优势明显;另一方面，分子诊断可以实现在 ...

Mullis used the basis of Sanger's DNA sequencing as a starting point for his new technique. He realized that the repeated use of DNA polymerase triggered a chain reaction resulting in a specific DNA ...

A polymerase chain reaction (PCR) test detects genetic material from a pathogen or abnormal cell sample. Ways of collecting samples include a nasal swab, a saliva swab, or taking a sample of blood.

RT-qPCR stands for quantitative reverse transcription polymerase chain reaction, the “RT” not to be mistaken for “real time”. 2 Unlike qPCR that uses a DNA template, the starting material for RT-qPCR ...

PURPOSE: Previous reports have indicated that reverse transcriptase polymerase chain reaction (RT-PCR) for cytokeratin 19 (CK-19) may be useful in the management of patients with breast cancer.

Shorter-Duration Therapy Using Vincristine, Dactinomycin, and Lower-Dose Cyclophosphamide With or Without Radiotherapy for Patients With Newly Diagnosed Low-Risk Rhabdomyosarcoma: A Report From the ...