这篇研究通过创新的巢式PCR（nested PCR）两步扩增策略，显著提升了rpoB基因标记在低浓度细菌DNA和宿主相关微生物组（host-associated microbiota）宏条形码（metabarcoding）分析中的灵敏度。该方法克服了传统单步PCR因引物高度简并性导致的扩增效率低下问题，通过体外 ...

PCR——实验室必备实验，从基因鉴定到信号通路验证，几乎每周都要做那么几次。 然而，各种PCR技术，包括PCR、qPCR、RT-PCR、RT-qPCR、Real-Time PCR和dPCR，长得实在是太像了，好多同学看得头晕眼花也没弄明白这几种PCR有什么区别。 今天，一篇文章，带你清清楚楚 ...

Real-time PCR has many advantages compared with antigenemia and qualitative PCR assays for detecting cytomegalovirus (CMV) infection in patients following SCT. However, the procedure used in each ...

Legionnaires' disease (LD) is a globally distributed systemic infectious disease. The burden of LD in many regions is still unclear, especially in Asian countries including China. A survey of ...