We describe a protocol that uses a bioinformatically optimized primer in an isothermal whole genome amplification (WGA) reaction. Overnight incubation at 37 °C efficiently generates several hundred- ...

Next-generation sequencing (NGS) revolutionized the life sciences and has given rise to fields such as clinical sequencing, advanced our understanding of genome-typing and gene expression, and allowed ...

Introducing our In Focus on target enrichment for next-generation sequencing (NGS), this video describes the importance of NGS in life science research, reviews available techniques for enriching ...

Next-generation sequencing (NGS) target enrichment methods provide solutions to focus on and sequence a small portion of the human genome. This saves sequencing and analysis costs for labs that study ...

Target enrichment workflows for Next Generation Sequencing are well-established methods that enable labs to achieve relatively low sequencing costs by specifically sequencing regions of interest.

Proceedings of the National Academy of Sciences of the United States of America, Vol. 113, No. 21 (May 24, 2016), pp. 5916-5921 (6 pages) DNA replication occurs semidiscontinuously due to the ...