A plasmid is a small, circular, double-stranded DNA molecule that is distinct from a cell's chromosomal DNA. Plasmids naturally exist in bacterial cells, and they also occur in some eukaryotes. Often, ...

Molecular biology is an ever-changing field, and most significant developments have happened because of changes in plasmid DNA sequencing. A plasmid, a circular and small double-stranded DNA entity ...

Tuberculosis (TB) remains a threat for public health, killing around 3 million people a year. Despite the fact that most cases can be cured with antibiotics, the treatment is long and patients relapse ...

Bacterial plasmids are circular or linear double-stranded DNA molecules defined by their capability of autonomous replication in the hosts. They are critical sources for microbial evolution and genome ...

A hybrid bacteriophage, P420, was constructed in vitro; it contains part of bacteriophage P4 and a 3.6-kilobase derivative of plasmid ColE1. In Escherichia coli, the plasmid-phage hybrid can exist as ...

For Research Use Only. Not for use in diagnostic procedures. The purification of plasmid DNA (pDNA) is essential in molecular biology research. Over the years, plasmid DNA isolation technology has ...

Plasmid DNA isolation from bacteria is a vital molecular biology method used to create template DNA for preferred downstream reactions. Plasmid isolation approaches are basic; however, high-throughput ...

PT181 is a naturally occurring 4.5-kilobase Staphylococcus aureus plasmid encoding resistance to tetracycline. The plasmid has a copy number of about 20 per cell; a mutant, cop-608, that has a copy ...

In the development of DNA vaccines and cell and gene therapies, manufacturing plasmid DNA creates a crucial bottleneck. Such a slowdown could intensify with the market for manufacturing DNA plasmids ...

For manufacturing plasmid DNA, good manufacturing practice begins with prudent vector design (Figure 1). While the eukaryotic promoter, the gene sequence, and the poly-A site primarily affect the ...