Ideally, gene expression studies by quantitative RT-PCR (qRT-PCR) should be performed using RNA from homogeneous cell populations. Frequently, this means performing qRT-PCR using picogram amounts of ...

This is a preview. Log in through your library . Abstract The use of new powerful nested polymerase chain reaction (PCR) techniques to identify and screen for prevalence of parasites has a huge ...

Figure 2: Tetraplasmic distribution of alleles is confirmed using pre-PCR separation by gel electrophoresis, which is not subject to PCR artifacts. We carried out two additional experiments to exclude ...

Combining multiplex PCR, sequentially, with reverse line blot hybridization (mPCR/RLB) is a convenient, objective way to identify up to 43 targets in 43 individual specimens simultaneously (using a 45 ...

Advanced therapy medicinal products (ATMPs) demand rigorous sterility testing. Yet traditional growth-based methods require 14 days for results, creating a critical bottleneck for short shelf-life ...

High-resolution RNA analysis using next-generation sequencing (RNA-seq) offers a comprehensive assessment of the transcriptome, allowing for quantification of global gene expression. The utility of ...